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Gram+ Gram− Atypical

Gram Stain Organism Lookup

Search by organism name or ICD-10 code. Find Gram classification, morphology, and coding guidance from culture results.

How Gram Stain Affects ICD-10 Coding

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Gram-Positive (+)

Cell wall retains crystal violet dye → stains purple under microscope. Most B95 codes (Staph, Strep, Enterococcus) are Gram-positive. Key sepsis codes: A41.01 (MRSA sepsis), A41.02 (MSSA sepsis), A41.0 (Staph sepsis).

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Gram-Negative (−)

Thin cell wall does not retain dye → stains pink/red. Most B96 codes are Gram-negative. Sepsis from Gram-negative organisms → A41.5x range. Gram-negative bacteria are generally harder to treat — document organism specifically when culture is available.

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Atypical / No Cell Wall

Organisms like Mycoplasma and Chlamydia lack a peptidoglycan cell wall — they don't Gram stain. Coded separately (B96.0 for Mycoplasma pneumoniae). Spirochetes (Borrelia, Treponema) stain poorly and are considered Gram-negative by convention.

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Anaerobes

Can be Gram+ or Gram−. Bacteroides fragilis (B96.6) is Gram-negative anaerobic — common in abdominal/pelvic infections. Clostridium perfringens (B96.7) is Gram-positive anaerobic — gas gangrene, necrotizing fasciitis. Document "anaerobic" in the culture result for specificity.

⚡ Coding Tip: B95–B97 codes are always secondary — they identify the causative organism for a condition coded elsewhere (e.g. pneumonia, UTI, sepsis). Never code B95–B97 as a principal diagnosis. When the culture identifies the organism, use the most specific code available — do not default to "unspecified."